Journal: Molecular neurobiology
Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood-Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats.
doi: 10.1007/s12035-024-04337-8
Figure Lengend Snippet: Fig. 2 The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A, B, C, D, E, F The NGF + SMES group had signifi- cant effect except PrL and MS. The results are demonstrated as the mean ± SEM (n = 5, each group).*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are dem- onstrated as the mean ± SEM (n = 5, each group). **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. hippocampus group. a, b, c, d, e, f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM (n = 5 or 8, each group). *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. NGF + SMES group. g The hip- pocampus had significant effect. The results are demonstrated as the mean ± SEM (n = 6, each group). ****P < 0.0001 vs. hip- pocampus group
Article Snippet: Enzyme‐Linked Immunosorbent Assay Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse BetaNGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).
Techniques: Expressing, Enzyme-linked Immunosorbent Assay